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KMID : 0355220070320040357
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Journal of Korean Academy Oral Medicine
2007 Volume.32 No. 4 p.357 ~ p.364
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Effect of Glucose at High Concentrations on the Apoptosis of the Cultured Periodontal Ligament Cells and Osteoblasts
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Park Sung-Ho
Jue Seong-Suk
Hong Jung-Pyo
Shin Je-Won
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Abstract
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This experiment was designed to clarify the effect of extracellular glucose on the osteoblasts and periodontal ligament cells. The cells were incubated for 24 and 48 hours with α-MEM including 1,000 ㎎/ℓ (control group) and 4,500 ㎎/ℓ (experimental group) of glucose. Then, the expressions of caspase-3, p38 MAPK, JNK-1, and ERK-1 were examined using Elisa assay and Western blot. The results were as follows:
1. The expression of caspase-3 and p38 MAPK was increased by the high extracellular glucose in both cells.
2. The expression of caspase-3 and p38 MAPK was increased greatly in the periodontal ligament cells than the E1 cells by the high extracellular glucose.
3. The expression of JNK-1 was increased by the high extracellular glucose in both cells.
4. The expression of ERK-1 was not changed by the high extracellular glucose in both cells.
These results suggest that extracellular glucose at high concentrations may inhibit the periodontal regeneration process increasing cellular apoptosis. And p38 MAPK and JNK-1 pathway may be the most responsible intracellular pathway rather than ERK-1.
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KEYWORD
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p38 MAPK, JNK-1, Glucose, Osteoblast, Periodontal ligament cell, Apoptosis
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